To watch bleaching, we use the “Trigger component positions at specific frames” function of Acquire > Stream Acquisition...
MetaMorph’s uber powerful Multidimensional Acquisition (MDA) module is does not allow running journals (and thus using the Mosaic) during a stream; only at he beginning or end.
- Draw your region(s).
- Save your regions by clicking “Active Region” in the Location tab of the Targeted Illumination window, and then click “Update Device Mask.
- Click “LatestMask” to complete programming the Mosaic.
- Make sure the Component is set to “Photonic Instruments Mosaic Mask” and the Position to “Pulse”.
- Set the trigger time point to pulse the Mosaic and add the “Trigger move”.